Summary of Study ST000040
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000018. The data can be accessed directly via it's Project DOI: 10.21228/M8Z59Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000040 |
| Study Title | Heatshock response of C. elegans using IROA (I) |
| Institute | University of Florida |
| Department | Biochemistry & Molecular Biology |
| Laboratory | Edison |
| Last Name | Stupp |
| First Name | Gregory |
| stuppie@ufl.edu | |
| Submit Date | 2013-12-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Uploaded File Size | 3.2 G |
| Analysis Type Detail | LC-MS |
| Release Date | 2014-03-05 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP000053 |
| Sampleprep Summary: | Supernatant (endometabolome) was separated from worm pellets (exometabolome) by centrifugation. The supernatant was filtered, lyophilized, and resuspended in 100 μL of LC-MS grade H2O. Worm pellet (endometabolome)was separated from supernatant (exometabolome) by centrifugation. The worm pellets were homogenized using a Biospec Mini-Beadbeater-8 in 80% methanol, dried under nitrogen and resuspended in 100 μL of LC-MS grade H2O. |
| Processing Method: | filtration with 0.2 micron nitrocellulose, frozen at -80C, lyophilized and resuspended in 100 μL of LC-MS grade H2O. homogenization with Biospec Mini-Beadbeater-8 in 80% methanol, dried under nitrogen and resuspended in 100 μL of LC-MS grade H2O. |
